qPCR Talks
BioEPS
is presenting qPCR 2011 eConference online via video
stream!
With our new eSeminars
and eConference tool a
world-wide know-how transfer becomes possible in a fast and simple way.
The qPCR 2010 package
contains 50 talks from our qPCR Symposium
2010 in Vienna
Topics: MIQE guidelines, High throughput
qPCR,
RNAi-miRNA, qPCR Data analysis, HRM, Circulating
Nucleic Acids, Single Cell qPCR, and more ... ...
The qPCR 2011 package
contains 65 talks from our qPCR Symposium
2011 in Freising-Weihenstephan
Topics: Single Cell qPCR,
Next Generation Sequencing, High throughput
qPCR, MIQE guidelines, RNAi-miRNA,
qPCR Data analysis, HRM, Circulating
Nucleic Acids, Pre-analytical Steps, New qPCR Applications in
Molecular Diagnostics, and more ... ...
To
get connected - Visit
our
web shop => http://eConference.bioEPS.com
Access the complete eConference qPCR 2010 packages
contains 50 talks in high
resolution from the qPCR Symposium 2010 in Vienna with all sessions
Access
the complete eConference qPCR 2011 packages
contains 65 talks in high
resolution from the qPCR Symposium 2011 in Freising-Weihenstephan with
all sessions
qPCR
2009 - Proceedings,
Talks and Posters are free for
download !
qPCR
2007 - Symposium Presentations
All scientific
contributions are published in the qPCR
2007 Symposium Proceedings
Download
the talks
and poster presentations from previous qPCR
Symposia
RefGenes - a unique tool to find suitable reference genes
Interpretation requires context - making sense out of
gene lists and networks
RefGenes was presented at the 2011 qPCR Conference, Weihenstephan,
Germany
RefGenes: identification of reliable
and condition specific reference genes for RT-qPCR data normalization.
Hruz T, Wyss M, Docquier M, Pfaffl MW, Masanetz S,
Borghi L, Verbrugge P, Kalaydjieva L, Bleuler S, Laule O, Descombes P,
Gruissem W and P Zimmermann
BMC Genomics 2011, 12: 156
General
problem
The choice of suitable reference genes is absolutely crucial in RT-qPCR
gene expression analysis. Often, genes from commercial panels don't
work well for one's own biological context. Ideally, the expression of
reference genes should remain unchanged across samples within the
context under study.
Solution
RefGenes is an online app from Genevestigator that
allows users to search for genes that are most stable across a chosen
set of samples based on microarray data. This set of samples can be
chosen according to experimental conditions or tissue types. For
example, if you are performing a RT-qPCR experiment on mouse liver
samples, you can use RefGenes to identify the set of genes that are
most stable across all microarrays done on mouse liver in
Genevestigator. This method offers two major improvements over existing
methods because a) it does not narrow down from a small set of genes
(e.g. commercial housekeeping gene panels), but looks for novel
candidates from a genome-wide set of genes b) it is based on
condition-specific stability. The below schema shows how RefGenes can
be used in combination with existing approaches to yield valuable
reference genes for specific experimental conditions.
- Some Talks from the qPCR
Meeting in London 2003,
organized by Stephen Bustin:
- Talks
by J.M. Gallup, Department of Veterinary Pathology, College of
Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1250, USA.:
- Nice
talks presentations from David Wishart, Departments of Computing
Science and Biological Sciences, University of Alberta,
Edmonton, Alberta; USA:
- Determination
of real-time PCR efficiency - An overview
held
at
the
qPCR
2004
Event download
PDF
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