Available
real-time PCR cyclers (3)
Corbett
Life Science: Rotor-Gene 6000 with HRM
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The
Rotor-Gene 6000 is a third-generation real-time rotary analyzer. Its
unique
centrifugal format was designed specifically for the demanding
requirements
of real-time cycling and thermo-optical analyses.
The rotary design provides unparalleled well-to-well thermal and
optical uniformity—critical factors in real-time analysis. Its also
enables a fast data acquisition rate, a feature now proving to be
essential for advanced applications such as HRM (high-resolution melt).
In short, the superior performance of the Rotor-Gene 6000 delivers
versatility:
- It
is the only real-time amplification platform capable of HRM
(high-resolution melt) and nucleic acid concentration measurement in
addition to all the
standard real-time applications like quantitative amplification and SNP
genotyping.
- It
has the greatest optical range, with 6 channels spanning UV to
infra-red wavelengths.
- It
uses the widest range of interchangeable reaction tube formats.
With the
Rotor-Gene 6000 you can embrace or invent the future of real-time
analysis and unlock new research possibilities. MORE… |
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Open Chemistry Platform
The Rotor-Gene multi-filter system can detect all
available real time chemistries including SybrGreen, Taqman,
FRET and Molecular Beacons. Most DNA
amplification enzymes/buffers can be used on the
system to generate Quantitaion/Melt data. It
is not necessary to use expensive kits
that are specific to the instrument.
User Friendly Software Interface
The Rotor-Gene offers the most user friendly real
time analysis software system currently available. Developed over the
past three years based on customer feedback worldwide, the software has
been refined to the point where it is now recognized as one
of
the most powerful yet user friendly packages available.
Download
Software Upgrades
All Rotor-Gene
users are given free access to software upgrades
which can be downloaded from our website. There is
also a registered users group where information
can be shared and suggestions made to further improve
or customise the software.
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LTF Labortechnik
is the exclusive RotorGene distributors in Germany.
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SmartCycler II & GeneXpert
(Cepheid)
 
The
"heart" of the Smart Cycler® System from Cepheid of Sunnyvale,
Calif., is the I-CORE™ (Intelligent Cooling/Heating Optical Reaction)
module. According to company literature, the I-CORE module incorporates
state-of-the-art microfluidic and microelectronic design. Each Smart
Cycler processing block contains 16 independently programmable I-CORE
modules, each of which performs four-color, real-time fluorometric
detection.
A wide variety of different multiplex or simplex fluorescent tags can
used in conjunction with this system, including FAM, TET, ALEXA 532, ALEXA 647,
TAM,
ROX, SYBR Green, Cy3, Texas Red and others.
Main
features of the SmartCycler II system:
•
Expandable:
16, 32, 48, 64, 80 or 96 independent reaction sites
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Flexible
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Fast
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Real-time detection
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Four-channel optics
The
GeneXpert® System utilizes real-time polymerase chain reaction
(PCR) to amplify and detect target DNA. The system is the first to
fully automate and integrate all the steps required for PCR-based DNA
testing: sample preparation, DNA amplification and detection.
Self Contained Cartridges
Integrated sample preparation - for automated and self-contained
testing, features and benefits:
- Reagent
containment and delivery
- Sample
and reagent aliquoting and mixing; continuous flow for large volume
handling
- Cell
separation and concentration using modular filter and solid-phase
holder assemblies
- Rapid
cell lysis using ultrasonic techniques
- DNA or
RNA capture, enrichment, purification using chips or solid-phase
materials
- Preparing
reaction mixture and filling integrated PCR reaction tube
- The
cartridges are designed for use with Cepheid's real-time modules, thus
fully integrating the steps associated with DNA extraction,
amplification, and detection in one system and one procedure.
The
Mx™ Family of qPCR Systems from Stratagene offers the highest
performance and flexibility in qPCR Systems at the most affordable
price. The Mx3000P® qPCR System is not just an entry-level QPCR
system, but is a high-performance, full-featured instrument system
designed to accommodate most experimental designs. The new Mx3005P™
qPCR System advances the proven Mx3000P system by offering unmatched
flexibility and capability to support more real-time qPCR applications
and chemistries. The flexible Mx3005P system will accommodate your
research needs now and in the future.
Mx3000P® qPCR System
The
Mx3000P® qPCR System is the first qPCR system with features to
support a wide-range of applications, qPCR chemistries and reagents.
The four-color system includes the flexibility required for more
advanced applications, but is affordable for the individual researcher
on a limited budget.
Mx3005P™ qPCR System
The
new Mx3005P® qPCR System includes expanded features to support a
wider range of real-time PCR applications, such as simultaneous
five-target detection and support for alternative qPCR probe
chemistries. The five-color optical system with flexible filter
wheel channel control expands the utility of Mx30005P system into new
and emerging applications and chemistries.
Mx3000P® and Mx3005P™
qPCR System Features
Instrument Features:
- Excitation:
Quartz tungsten-halogen lamp via scanning fiberoptics
- Detector:
Single photomultiplier tube (PMT) via scanning fiberoptics
- Filters:
Customizable filter wheels with four (Mx3000P® system) or five
(Mx3005P™ system) user-selected filter sets
- Filter
control: Custom filter path selection to support FRET signal detection
(Mx3005P™ system only)
- Thermal
system: Solid state, Peltier-based, 96-well block thermal system
- Sample
format: Standard 96-well plates, 8-strip tubes, 200-µl tubes,
optimized for 25-µl reactions
- Embedded
computer collects and saves experiment data during power failure or
computer crash
- Dimensions:
13" (33 cm) W x 18" (46 cm) D x 17" (43 cm) H, Weight: 45 lbs. (20.4 kg)
- Power:
100 to 230 VAC +/- 10%, 50/60 Hz
System Performance:
- Linear
dynamic range: 10 orders of magnitude, standard curve method
- Excitation
range: 350 to 750 nm
- Emission
range: 350 to 700 nm
- Temperature
uniformity: +/- 0.25°C at 72°C
- Thermal
block ramp rate: Up to 2.5°C/second
- Amplification
rate: Standard 40-cycle, two-step QPCR reaction completed in 90
minutes; reaction is completed in 60 minutes using FullVelocity™
SYBR® Green QPCR reagents (without dissociation curve)
- Discrimination:
Distinguish samples with 5,000 and 10,000 template copies with a 99.7%
confidence level
- Detection
down to single-copy equivalents
New MxPro™ QPCR Software:
- Automatically
detects and interfaces with Mx3000P (4-color) or Mx3005P system
(5-color) system
- Interfaces
with Mx3000P and Mx3005P systems simultaneously and supports up to six
systems in any combination attached to a single computer
- Streamlined
plate setup with dye editing dropdown menu
- Right
mouse-click capability throughout the software to allow quicker and
easier access to menu functions
- The
Comparative Quantification module generates and displays normalized
fold change values on a chart using a base 2 logarithmic scale.
- Gene
expression fold change is displayed with upper and lower limit error
bars based on replicate variability for up to four targets normalized
to a single reference gene with efficiency corrections for each gene
target
- View
amplification plots and standard curves for up to five targets on the
same screen with threshold changes instantly updated on the standard
curve
- Improved
text reports display target-specific data analysis parameters at the
top of each report and allow for customization through adding/removing
columns and enhanced sorting capability
- Export
any data set directly into .xls, .ppt, .bmp, .txt, or .xml format.
Charts and graphs can be exported directly to Microsoft®
PowerPoint® and Excel®
- Auto
generation of charts and graphs in Excel during export
- Create
custom consolidated data reports by selecting which analysis screens
to include and how the report will organize the data
Choice of Filters:
- ALEXA
Fluor® 350 (350 - 440 nm)
- FAM™/SYBR®
Green I (492 - 516 nm)
- TET™
(517 - 538 nm)
- HEX™/JOE™/VIC™
(535 - 555 nm)
- Cy™3
(545 - 568 nm)
- TAMRA™
(556 - 580 nm)
- ROX™/Texas
Red® (585 - 610 nm)
- Cy™5
(635 - 665 nm)
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