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Available
real-time PCR cyclers (3)
Available
real-time PCR cyclers (1)
Available
real-time PCR cyclers (2)
Available
real-time PCR cyclers (4)
Qiagen -
Rotor-Gene Q with HRM
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Real-time PCR detection - Rotor-Gene Q - pure
detection
QIAGEN’s new real-time PCR cycler, the Rotor-Gene Q, provides
outstanding performance and the reliable and accurate results your
research demands. Together with optimized QIAGEN kits, the Rotor-Gene Q
enables streamlined analysis for a wide range of applications.
* Outstanding thermal and optical performance
* More applications than any other real-time cycler
* Unmatched optical range with up to 6 channels
* Robust design enables easy assay setup and minimal
maintenance
* Compatible with the QIAgility for automated PCR
setup
The
unique rotary design
delivers:
* Well-to-well variations below ±0.01°C
(20 times lower than block cyclers)
* Uniform detection eliminating the need for ROX
reference dye
* Fast ramping and negligible equilibration times
for short run times
* Complete confidence in your results!
Comprehensive
application
range
The Rotor-Gene Q combined with optimized QIAGEN real-time PCR kits and
assays offer reliable results in all real-time PCR and high-resolution
melt applications, including:
* Gene expression analysis
* Pathogen detection
* DNA methylation analysis
* Genotyping
* Mutation scanning
* High Resolution Melting analysis
Rotor-Gene ScreenClust HRM Software for highly effective high-resolution melting
analysis
* Innovative mathematical approach to HRM analysis
* Highly accurate identification of genotypes in
supervised mode
* Automatic detection of new mutations in
unsupervised mode
* Robust statistics for classifying and interpreting
HRM data
* Minimal effort and standardized processes for data
interpretation
HRM - an
innovative technology for analysis of genetic variations
View and listen to this seminar
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Principle and procedure - Unique rotary
design for outstanding performance
The unique centrifugal rotary design of the Rotor-Gene Q makes it the
most precise and versatile real-time PCR cycler currently available
(see figure "Cross-section of the Rotor-Gene Q"). Each tube spins in a
chamber of moving air, keeping all samples at precisely the same
temperature during rapid thermal cycling. Detection is similarly
uniform. When each tube aligns with the detection optics, the sample is
illuminated and the fluorescent signal is rapidly collected from a
single, short optical pathway. This thermal and optical uniformity
results in sensitive, precise, and fast real-time PCR analysis (see
figure "Precise real-time PCR analysis"). It also eliminates
sample-to-sample variations and edge effects. These are unavoidable in
traditional block-based instruments due to temperature gradients across
the block and multiple, complex optical pathways.
The rotary design delivers:
* Well-to-well variation below ±0.01°C
(20 times less than block cyclers)
* Uniform detection eliminating the need for ROX
reference dye
* Fast ramping and negligible equilibration times
for short run times
* Complete confidence in your results!
Unrivaled optical range
enables multiple applications
Whether your assay is based on intercalating dyes such as SYBR Green,
probes such as hydrolysis (TaqMan) or hybridization (FRET) probes, or
multiplex chemistries, the Rotor-Gene Q meets your requirements. With
up to 6 channels spanning UV to infrared wavelengths, the cycler
delivers the widest optical range currently available (see Table
"Channels for optical detection"). In addition, the software allows you
to create new excitation/detection wavelength combinations, which means
that the Rotor-Gene Q is compatible with dyes you may use in the
future.
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REST 2009 Software
For gene expression analysis using real-time PCR data from the
Rotor-Gene Q and other cyclers
* Estimates up and down regulation for gene
expression studies
* Analysis using randomization and bootstrapping
techniques
* Graphical data output via whisker-box plots
REST 2009 Software is a standalone software tool, developed by M.
Pfaffl (Technical University Munich) and QIAGEN, for analysis of gene
expression data from quantitative real-time PCR experiments. Click the
"Resources" tab to download REST 2009 Software free of charge.
Download
newest
version REST 2009 => http://www.REST.de.com
Download User Guide => REST
2009 Software User Guide - English (PDF)
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The
Rotor-Gene 6000 is a third-generation real-time rotary analyzer. Its
unique
centrifugal format was designed specifically for the demanding
requirements
of real-time cycling and thermo-optical analyses.
The rotary design provides unparalleled well-to-well thermal and
optical uniformity—critical factors in real-time analysis. Its also
enables a fast data acquisition rate, a feature now proving to be
essential for advanced applications such as HRM (high-resolution melt).
In short, the superior performance of the Rotor-Gene 6000 delivers
versatility:
- It
is the only real-time amplification platform capable of HRM
(high-resolution melt) and nucleic acid concentration measurement in
addition to all the
standard real-time applications like quantitative amplification and SNP
genotyping.
- It
has the greatest optical range, with 6 channels spanning UV to
infra-red wavelengths.
- It
uses the widest range of interchangeable reaction tube formats.
With the
Rotor-Gene 6000 you can embrace or invent the future of real-time
analysis and unlock new research possibilities. MORE… |
SmartCycler II &
GeneXpert
(Cepheid)
 
The
"heart" of the Smart Cycler® System from Cepheid of Sunnyvale,
Calif., is the I-CORE™ (Intelligent Cooling/Heating Optical Reaction)
module. According to company literature, the I-CORE module incorporates
state-of-the-art microfluidic and microelectronic design. Each Smart
Cycler processing block contains 16 independently programmable I-CORE
modules, each of which performs four-color, real-time fluorometric
detection.
A wide variety of different multiplex or simplex fluorescent tags can
used in conjunction with this system, including FAM, TET, ALEXA
532, ALEXA 647, TAM,
ROX, SYBR Green, Cy3, Texas Red and others.
Main
features of the SmartCycler II system:
•
Expandable:
16, 32, 48, 64, 80 or 96 independent reaction sites
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Flexible
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Fast
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Real-time detection
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Four-channel optics
The
GeneXpert® System utilizes real-time polymerase chain reaction
(PCR) to amplify and detect target DNA. The system is the first to
fully automate and integrate all the steps required for PCR-based DNA
testing: sample preparation, DNA amplification and detection.
Self Contained Cartridges
Integrated sample preparation - for automated and self-contained
testing, features and benefits:
- Reagent
containment and delivery
- Sample
and reagent aliquoting and mixing; continuous flow for large volume
handling
- Cell
separation and concentration using modular filter and solid-phase
holder assemblies
- Rapid
cell lysis using ultrasonic techniques
- DNA or
RNA capture, enrichment, purification using chips or solid-phase
materials
- Preparing
reaction mixture and filling integrated PCR reaction tube
- The
cartridges are designed for use with Cepheid's real-time modules, thus
fully integrating the steps associated with DNA extraction,
amplification, and detection in one system and one procedure.
The
Mx™ Family of qPCR Systems from Stratagene offers the highest
performance and flexibility in qPCR Systems at the most affordable
price. The Mx3000P® qPCR System is not just an entry-level QPCR
system, but is a high-performance, full-featured instrument system
designed to accommodate most experimental designs. The new Mx3005P™
qPCR System advances the proven Mx3000P system by offering unmatched
flexibility and capability to support more real-time qPCR applications
and chemistries. The flexible Mx3005P system will accommodate your
research needs now and in the future.
Mx3000P® qPCR System
Cited
in more than
3000 publications, the Agilent Mx3005P and Mx3000P QPCR Systems are the
most flexible—and reliable—instruments available for gene expression
analysis, microarray data validation, SNP genotyping, pathogen
detection, DNA methylation analysis, and chromatin immuno-precipitation
studies. Agilent’s qPCR software, MxPro, provides users with an
intuitive interface, quick experiment design, powerful data analysis
and easy report generation. All of these features and more make qPCR
with the Mx instrument an exciting and dependable user experience.
Mx3005P™ qPCR System
Cited
in more than 3000 publications, the Agilent Mx3005P and Mx3000P QPCR
Systems are the most flexible—and reliable—instruments available for
gene expression analysis, microarray data validation, SNP genotyping,
pathogen detection, DNA methylation analysis, and chromatin
immuno-precipitation studies. Agilent’s qPCR software, MxPro, provides
users with an intuitive interface, quick experiment design, powerful
data analysis and easy report generation. All of these features and
more make qPCR with the Mx instrument an exciting and dependable user
experience.
MXPro ET QPCR Software
Mx Pro QPCR software with FDA guideline 21 CFR Part 11 compatible
features. Four key elements have been added to create the ET software
version; secure application login, database file management, electronic
audit trail, and report generation.
Mx3000P®
and Mx3005P™
qPCR System Features
Instrument Features:
- Excitation:
Quartz tungsten-halogen lamp via scanning fiberoptics
- Detector:
Single photomultiplier tube (PMT) via scanning fiberoptics
- Filters:
Customizable filter wheels with four (Mx3000P® system) or five
(Mx3005P™ system) user-selected filter sets
- Filter
control: Custom filter path selection to support FRET signal detection
(Mx3005P™ system only)
- Thermal
system: Solid state, Peltier-based, 96-well block thermal system
- Sample
format: Standard 96-well plates, 8-strip tubes, 200-µl tubes,
optimized for 25-µl reactions
- Embedded
computer collects and saves experiment data during power failure or
computer crash
- Dimensions:
13" (33 cm) W x 18" (46 cm) D x 17" (43 cm) H, Weight: 45 lbs. (20.4 kg)
- Power:
100 to 230 VAC +/- 10%, 50/60 Hz
System
Performance:
- Linear
dynamic range: 10 orders of magnitude, standard curve method
- Excitation
range: 350 to 750 nm
- Emission
range: 350 to 700 nm
- Temperature
uniformity: +/- 0.25°C at 72°C
- Thermal
block ramp rate: Up to 2.5°C/second
- Amplification
rate: Standard 40-cycle, two-step QPCR reaction completed in 90
minutes; reaction is completed in 60 minutes using FullVelocity™
SYBR® Green QPCR reagents (without dissociation curve)
- Discrimination:
Distinguish samples with 5,000 and 10,000 template copies with a 99.7%
confidence level
- Detection
down to single-copy equivalents
MXPro ET QPCR Software
- Automatically
detects and interfaces with Mx3000P (4-color) or Mx3005P system
(5-color) system
- Interfaces
with Mx3000P and Mx3005P systems simultaneously and supports up to six
systems in any combination attached to a single computer
- Streamlined
plate setup with dye editing dropdown menu
- Right
mouse-click capability throughout the software to allow quicker and
easier access to menu functions
- The
Comparative Quantification module generates and displays normalized
fold change values on a chart using a base 2 logarithmic scale.
- Gene
expression fold change is displayed with upper and lower limit error
bars based on replicate variability for up to four targets normalized
to a single reference gene with efficiency corrections for each gene
target
- View
amplification plots and standard curves for up to five targets on the
same screen with threshold changes instantly updated on the standard
curve
- Improved
text reports display target-specific data analysis parameters at the
top of each report and allow for customization through adding/removing
columns and enhanced sorting capability
- Export
any data set directly into .xls, .ppt, .bmp, .txt, or .xml format.
Charts and graphs can be exported directly to Microsoft®
PowerPoint® and Excel®
- Auto
generation of charts and graphs in Excel during export
- Create
custom consolidated data reports by selecting which analysis screens
to include and how the report will organize the data
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