Available real-time RT-PCR cycler and kinetic PCR machines

	Accompanying Quantica® is Techne’s unique and fully flexible, intuitive wizard based application software Quansoft.

BioGene Thermal Cycler Systems

Patent pending Integrated Heating Technology (IHT) forms the basis of this system. It has long been recognised that intra-sample variations caused by thermal gradients within metal block or air-heating systems causes differences in the final product (quantity and quality). IHT uses the concept of directly heating the vessel containing the reaction mix and in addition having a single, infrared temperature sensor for each vessel to ensure truly independent thermal control. Direct heating also has a significant effect on thermal transfer rates as does the ‘capillary’ design of the reaction vessel (allows for a high surface to volume ratio) both leading to Ultra-Rapid thermal cycling. High throughput, cleaner reaction products and the ability to run reactions with different thermal profiles are significant advantages of this technology e.g. running gradients for process optimisation, multiple different tests on the same sample in diagnostic applications where time to report results is critical and high throughput screening. Synchrom mannual synchron.pdf

Patented Electrically Conducting Polymer (ECP) Technology forms the basis of this system. It has long been recognised that intra-sample variations caused by thermal gradients within metal block or air-heating systems causes differences in the final product (quantity and quality).
ECP Technology uses the concept of directly heating the vessel containing the reaction mix and in addition having a single, infrared temperature sensor for each vessel to ensure truly independent thermal control. Direct heating also has a significant effect on thermal transfer rates as does the ‘capillary’ design of the reaction vessel (allows for a high surface to volume ratio) both leading to Ultra Rapid thermal cycling.
High throughput, cleaner reaction products and the ability to run reactions with different thermal profiles are significant advantages of this technology e.g. running gradients for process optimisation, multiple different tests on the same sample in diagnostic applications where time to report results is critical and high throughput screening.
InSyte uses leading edge technology to ensure that the thermal performance is matched by fast, sensitive optics. A compact, high lifetime (10,000 hour) solid-state laser provides an intense, well-collimated pure light source at 473 nm (compatible with all standard chemistries, dyes and dye sets). A multichannel PMT collects all emitted wavelengths between 520 and 720 nm-the system is not constrained by use of filter sets to detect fixed bands of emitted fluorescence. This allows the user to ‘see the full picture’! High scan rates (full plate read every 3 seconds) provide good data sets for use in subsequent analysis.
View InSyte technical document

*	ULTRA-RAPID THERMAL CYCLING
*	UNIQUE 'Electrically Conducting Polymer' (ECP) technology facilitates:
*	DIRECT heating to a specially designed tube to allow rapid thermal equilibration of reaction mix.
*	TRULY Independent temperature control of each tube of the 96-MTP. Choose to run completely different temperature profiles in each tube!
*	FAST SENSITIVE, photon counting optics.
*	COMPREHENSIVE DATA LOGGING/ANALYSES: Embedded Graphical Control System enables user-friendly empirical set up and complete logging/analyses of data.

Real-Time PCR Cycler "DT-322"

Equipment manufactured by "DNA-Technology" corporation

http://www.dna-technology.com/catalog/ob_dt322_en.shtml

Real-Time PCR Cycler "DT-322" is intended for a carrying out of polimerase chain reaction (PCR) with a detection of an accumulation of PCR products during an amplification (real -Time PCR). DT-322 allows to carry out the qualitative and quantitative PCR analysis excepting a stage of the electrophoresis of PCR products in an agarose gel. The device is designed for simultaneous analysis of up to 32 samples. A thermocycler and an optical system of a fluorescence detection are united in this device. All data of the last amplification are recording into a private memory of the PCR Cycler and can be read out even after a current interruption occurred during an amplification. After a restoring of a power supply the amplification program will be continued, and the moment of interruption, its duration and a block temperature in the moment of interruption will be recorded. A turning off or a hanging of external computer does not result in PCR termination since the process control is managed by the private memory of the device that does not depend on a computer.

Brochure and technical parameters (PDF)

TL998 - Real-time Quantitative PCR Detecting System

http://www.medtl.com/english/ckcp.asp?id=8

The TL998 System provides an efficiency and high-speed solution for PCR analysis. Real-time PCR delivers increased sensitivity and a wider dynamic range more rapidly than traditional PCR. TL998 gives you a choice of quantitation methods for gene expression. It is very important in curative evaluation. The TL998 system ideally suited to meet the high-throughput requirements of today's drug discovery process. The TL998 is useful for life science research, and it is innovative and pioneering products for the prevention, diagnosis, evaluation and monitoring of diseases.

		Line-Gene-K is the newest system in Bioer's range of PCR detection systems. It features more advanced implementations of Peltier and fiber optic technology and a new direct current power system. The new instruments share the Peltier effect and fiber optic design features that provide the exceptional accuracy and stability of the Line-Gene instruments.The new units offer improved uniformity,stability and accuracy by utilizing a constant-current power supply and an advanced multi-point temperature control module.The Line-Gene.K has improved temperature control and more uniform heating throughout the sample block to improve the accuracy of PCR detection. The Line-Gene.K series is available in a range of automated and manual models with the ability to handle one, two, three or four combinations of excitation and emission wavelengths. http://www.bioer.com.cn/en/shengming_line_gene_k.htm

Line Gene is a complete real-time PCR detection system that can detect and quantitate nucleic acid sequences by monitoring fluorescence value during the PCR process. With innovative design on hardware and software, the unit is an ideal tool for research use in the field of gene analysis. Features: • Long life blue LED light Source • Extremely sensitive PMT detection module • Sandwich thermo-electronic (TE) module • Easy yet powerful software • Data Export http://www.bioer.com.cn/en/shengming_33pcr.htm

Line Gene II Real-time PCR Detection System is up-to-date product based on Line-gene. By using the advanced optical fiber conducting technique, the unit has strong sensitivity and reliability when detects the fluorescence. At the same time, it inherits all merits of Line-gene so to realize speedy and accurate PCR detection. What's more, the powerful software provides multiple channels fluorescence detection with four kinds of dyes at the same time. And the testing result of samples can be analyzed and compared by groups so that many functions, such as absolute quantitative, comparative quantitative, SNPs and melting curve analysis, can be accomplished. Multiple Applications: TaqMan probe; SYBR Green I; Molecular beacons, FAM, HEX, TAMRA, TEXASRED, ROX and so on. http://www.bioer.com.cn/en/shengming_66pcr.htm

http://eng.bioneer.com/instruments_qpcr.htm

Exicycler^TM Real Time Thermal Block and its operation software is optimized for applications such as quantification of gene expression, genotyping, counting bacterial/viral loads, melt-curve analysis and validation of microarray results. Exicycler is also capable of measuring mutual interactions between two molecules, such as protein-protein and protein-ligand, using fluorescence resonance energy transfer (FRET). In addition, the sensitivity, accuracy and system integration-ready design of Exicycler makes it ideal for high-throughput analysis.